HPLC works next The fundamental principle of skinny layer chromatography or column chromatography, the place it's a stationary section as well as a cell period. The cell stage flows from the stationary stage and carries the factors in the mixture with it.
각각 다른 산업 분야에 대한 자세한 정보 및 다양한 카테고리는 다음 써모 피셔 사이언티픽 학습 센터에서 산업 및 응용 과학 페이지를 확인하세요.
측정 가능한 농도 범위는 컬럼에 의해서도 결정됩니다. 컬럼 충진제의 종류, 입자 지름, 컬럼의 크기에 따라 분리에 최적인 시료 주입량이 크게 다릅니다.
Reducing the level of acetonitrile and expanding the quantity of h2o inside the mobile will maximize retention situations, providing much more time for you to effect a separation.
The selection from the column style depends upon the physicochemical Homes in the analytes currently being divided.
カラム周辺の温度の変動によって溶出時間が安定せず再現性が悪くなる場合があるため、カラム温度を一定に保つために使用する。またカラム温度を分離条件のパラメーターの一つとして積極的に利用する場合もある。
Data Examination software package is important for interpreting the data attained with the detector. The computer software displays the chromatogram, which can be a plot of detector sign as opposed to time. Important knowledge details incorporate:
Increase a regarded volume of the antidepressant protriptyline, which serves as an inside standard, to every serum sample and to each exterior common. To get rid of matrix interferents, pass a 0.5-mL aliquot of every serum sample or common through a C18 good-stage extraction cartridge. After washing the cartridge to eliminate the interferents, elute the remaining constituents, including the analyte and the internal typical, by washing the cartridge with 0.
The detector within an HPLC system identifies and quantifies the separated analytes. Widespread detectors include ultraviolet (UV) detectors that measure analyte absorbance at certain wavelengths.
The size in the particles and also the mechanical energy on the packing resources are the two key factors that impact column packing. The particle is usually packed and dried if larger sized than 20 mm, but if scaled-down than 20 mm, it has to be suspended in the right solvent. The slurry is check here then packaged.
There are several ways of detecting in the event the ingredient is passed out on the column. One of the strategy is by UV gentle. Several compounds absorbs UV lights of assorted wavelengths. UV light-weight is shinned where by the ingredient handed out with the column.
Frequent cellular section modifiers like acids and bases might be additional to fine-tune the conversation amongst analytes and the column. These modifiers can:
특히 컬럼의 선정은 분석의 결과에 영향을 미치기에 신중하게 선택하여야 합니다.
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